ABSTRACT
Objective: To establish the HPLC fingerprint for effective quality control and scientific evaluation of Erigeron breviscapus. Methods: Separation was performed on a Zorbax SB-C18 column (150 mm × 4.6 mm, 5 μm) and the mobile phase was methanol-0.1% phosphoric acid with gradient elution. The flow rate at 1 mL/min, the column temperature at 30 oC, and the detection wavelength at 335 nm. A total of 19 batches of E. breviscapus and its related species were analyzed. Similarity evaluation combined with hierarchical clustering analysis (HCA) and principal components analysis (PCA) were used to evaluate the quality of herbs from different batches. Results: The HPLC fingerprint of E. breviscapus was established with 11 common peaks, and five peaks were identified. Similarities of the 19 batches of samples were 0.873-0.978. Two batches of samples from its related species were high similarity. These 19 batches of samples could be classified into three clusters. The PCA result was consistent with HCA. The comprehensive score of S5 was the highest and the quality was the best. There was possibility for using E. multiradiatus as herbs instead of E. breviscapus. Conclusion: The establishment of HPLC fingerprint and the recognition of chemical pattern can provide a more comprehensive reference for the quality control of herbs.
ABSTRACT
Objective: To study the chemical constituents in the branches and leaves of Abies ernestii var. salouenensis. Methods: Some chromatographic methods were applied to isolating pure compounds and their structures were elucidated by spectroscopic methods. Results: Fourteen compounds were obtained and identified as kaempferol (1), quercetin (2), quercetin-3-O-α-L-rhamnoside (3), syringetin-3-O-β-D-glucopyranoside (4), kaempferol-3-O-β-D-glucopyranoside (5), kaempferol-3-O-β-D - (3″, 6″-di-E-p-coumaroyl)-glucopyranoside (6), laricitrin-3-O-β-D-glucopyranoside (7), vitexin (8), quercetin-7-O-β-D-glucopyranoside (9), apigenin-7-O-β-D-glucopyranoside (10), isohemiphloin (11), naringenin (12), (-)-epigallocatechin (13), and (+)-catechin (14). Conclusion: All the compounds are isolated from this plant for the first time, and compounds 7-11 and 13 are firstly isolated from the plants in Abies Mill.